Lower tier testing

Tier 1 and Tier 2 extended laboratory studies conducted in accordance with ESCORT II guidelines.

  • Tier 1 acute testing on species Aphidius rhopalosiphi, a beneficial parasitoid wasp and the predatory mite Typhlodromus pyri.
  • Tier 2 extended laboratory tests on a full range of indicator species and the latest associated guidelines approved for tiered testing, including:

Ground-dwelling beneficials

  • Aliochara bilineata
  • Poecilus cupreus
  • Pardosa spp.

Leaf-dwelling beneficials

  • Chrysoperla carnea
  • Coccinella septempunctata
  • Orius laevigatus
  • Episyrphus balteatus

Parasitic Hymenoptera

  • Aphidius rhopalosiphi
  • Trichogramma cacoeciae


  • Typhlodromus pyri
  • Phytoseiidae

Other species available upon request

Soil macro-organism studies

i2LResearch offers a full range of soil macro-organism studies: Earthworm laboratory acute toxicity (OECD 207) and reproduction (OECD 222) tests with Eisenia fetida, reproduction testing with Collembola (OECD 232) and Hypoaspis mites (OECD 226).  In addition, we offer testing on Enchytraeid worms, Enchytraeus spp. (OECD 220) and dung flies, S. stercoraria and M. autumnalis (OECD 228).

Full-scale field testing

In addition to full-scale field testing with earthworms, Collembola and mites, i2LResearch also offers a range of full fauna field studies with non-target arthropods (NTAs) in a variety of agricultural crops, following ISO 11268-3 and ISO 23611-1 and 2.

Bee testing

i2LResearch offers the following tests on honey bees (Apis mellifera) and bumble bees (Bombus terrestris) for the side-effect evaluation of products:

  • Acute contact and oral laboratory testing (OECD 213/214 and EPA OCSPP 850.3020)
  • Honey bee larval toxicity (OECD 237 and 239)
  • Chronic oral and accumulative toxicity (EFSA 3295)
  • Semi-field bee testing (EPPO PP/170(4) and OCSPP 850.3040)


Non-target plant testing (NTPs)

We offer non-target plant testing on seedling emergence (OECD 208) and vegetative vigour (OECD 227).


Aquatic ecotoxicology

i2LReserach also offers the following aquatic ecotoxicology testing:

  • Pulmonate snails, Lymnea stagnalis. (OECD 243)
  • Prosobranch snails, P. antipodarum. (OECD 242)
  • Chironomus spp. Spiked water or sediment (OECD 233)
  • Aquatic plants, Myriophyllum spicatum (OECD 238)
  • Aquatic plants, Lemna spp. (OECD 221)


Residue/Succeesing crop studies

i2L Research is able to conduct residue and succeeding crop trials on a number of crops including, but not restricted to:
  • Winter and Spring OSR
  • Winter and Spring Cereals
  • Maize
  • Potatoes
  • Vineyards
  • Fruit orchards